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. 2020 Oct 16;37(6):691–702. doi: 10.1007/s10719-020-09953-9

Fig. 2.

Fig. 2

Schematic of the enzymatic redox reaction resulting in fluorescent compound resorufin that forms the detection mechanism of the glycosidase plate-based assay. Resorufin measured at excitation (Ex.) wavelength of 571 nm and emission (Em.) wavelength of 586 nm. [NAD+: Nicotinamide adenine dinucleotide (oxidized); NADH: Nicotinamide adenine dinucleotide (reduced)]