Fig. 3.

Linear range of quantification of the glycosidase plate-based assay for the quantification of terminal galactosylation on human IgG standards of known starting amounts. The terminal galactosylation are expressed as not normalized values (Red) and as values normalized to the measured IgG amounts in the sub-samples of assay (Blue). The line formula is representative of the linear range (solid trend linear) of the not normalized terminal galactosylation values. The normalized values for the lowest amount of IgG tested, 30 μg, was not calculable due to lack of measureable absorbance signals above background at 280 nm