Figure 1.

Synthesis, characterization, and cytotoxicity test of combinatorial libraries of lipidoid nanoparticles. (A) Chemical structures and synthetic routes employed for the preparation of OCholB-, O16B-, and OChol-tailed lipidoids. (B) TEM image of 77-OCholB LNPs. Scale bar = 200 nm. (C) Cell viability tests of OCholB, O16B, OChol, and Lpf2k nanoparticles against HeLa cells. N = 4, two-tailed student’s t-test, p < 0.05. Data points at 1.0 and 3.3 mg/L were collected 8 h after dose. Data points at 47 and 91 mg/L were collected 24 h after dose. All data points in this and other figures are shown as mean±SD.