Fig. 1.

Schematic overview of different transfection methods: integrated co-transfection a refers to mixing different IVT-mRNAs prior to complexation with carrier, whereas in parallel co-transfections b, IVT-mRNAs are complexed in particles and added to cells separately, and in successive transfections c, cells are transfected with two types of IVT-mRNA with a 24-h interval. d Cellular uptake of different ratios of the two types of mRNA (vertical axis) and of different doses of both IVT-mRNAs (horizontal axis) results in different color distribution and intensity and can be used as a key readout for this study