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. 2020 Nov 4;98(12):1767–1779. doi: 10.1007/s00109-020-01956-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Simultaneous transfection of macrophages with EGFP and mCherry coding IVT-mRNA with two different strategies. Fluorescent microscopy images (bar = 50 μm) a and flow cytometric density plots b depicting the fraction of double-positive cells in integrated versus parallel co-transfected macrophages for an mRNA dose of 125 ng mL⁻¹. Analogous measurements were performed for cells transfected with various mRNA doses c. All experiments were performed using a liposomal carrier (LipoMM); multiple comparison t test revealed significant difference between iCoTF and pCoTF for all evaluated mRNA doses (p < 0.05). No fluorescent signal-/double-positive event was observed for untransfected macrophages. Values are presented as mean ± SD, n ≥ 3. Error bars indicate SD. (iCoTF, integrated co-transfection; pCoTF, parallel co-transfection)