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Quantitative evaluation of IVT-mRNA transfection
| mRNA concentration (ng mL−1) |
1st transfection | 2nd transfection | ||
|---|---|---|---|---|
| % mCherry+ (of total cells) |
% EGFP+ (of total cells) |
% EGFP+ within mCherrry+ |
% EGFP+ within mCherry- |
|
| 660 | 99.8 ± 0.1 | 93.6 ± 2.3 | 93.8 ± 2.2 | Not reported |
| 330 | 95.7 ± 1.2 | 81.4 ± 4.1 | 82.3 ± 3.9 | 61.0 ± 2.5 |
| 110 | 69.7 ± 0.5 | 39.9 ± 6.1 | 41.8 ± 6.2 | 35.4 ± 6.2 |
| 33 | 28.4 ± 1.1 | 13.4 ± 0.5 | 15.4 ± 0.7 | 12.5 ± 0.8 |
