. 2020 Aug 10;146(12):3155–3163. doi: 10.1007/s00432-020-03351-2

Table 1.

Analysis of cell death and late apoptosis in response to TDO inhibition

	SK-Mel-28	HCT-8	HUVEC
Late apoptosis Ann⁺ PI⁺	6.65 ± 0.8	8.76 ± 2.56	4.14 ± 0.13	6.57 ± 0.43	28.9 ± 0.93	28.9 ± 1.62
Dead Ann⁻ PI⁺	0.41 ± 0.17	0.72 ± 0.36	0.06 ± 0.01	0.13 ± 0.02	1.93 ± 0.65	0.16 ± 0.05

SK-Mel-28

HCT-8

HUVEC

+ 680C91

Late apoptosis

Ann⁺ PI⁺

6.65 ± 0.8

8.76 ± 2.56

4.14 ± 0.13

6.57 ± 0.43

28.9 ± 0.93

28.9 ± 1.62

Dead

Ann⁻ PI⁺

0.41 ± 0.17

0.72 ± 0.36

0.06 ± 0.01

0.13 ± 0.02

1.93 ± 0.65

0.16 ± 0.05

Apoptosis was assessed for untreated and 680C91-treated cells (40 µM) by means of flow cytometry. Percentage of late apoptosis was calculated for cells Annexin V⁺/ PI⁺; cells dead as Annexin V⁻/PI⁺. C: control unstimulated cells. Mean ± SEM, n = 3