Fig. 2.

Effects of the overexpression of APOL1 WT, G1 and G2 Vs on the sphingolipid pattern of human podocytes. Cell sphingolipids were metabolically labelled at the steady state with [1-³H]sphingosine, separated by HPTLC, visualized by digital autoradiography and quantified with M3 software. Data are expressed as percentage with respect to Mock cells. Gangliotetraosylceramide (GA1), globotriaosylceramide (Gb₃Cer), globopentaosylceramide (Gb₅Cer), monosialosyl-globopentaosylceramide (MSGb₅Cer), disialosyl-globopentaosylceramide (DSGb₅Cer), ceramide (Cer), glucosylceramide (GlcCer), lactosylceramide (LacCer), sphingomyelin (SM). *p < 0.05 vs Mock, **p < 0.005 vs Mock, °° p < 0.005 vs WT