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. 2020 Nov 17;37(6):713–727. doi: 10.1007/s10719-020-09963-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — PKCα translocation in lipid rafts microdomains. N2a cells were incubated in the absence (CTRL), or in the presence of 50 µM OligoGM1 for 3 h at 37 °C. Cells were subsequently subjected to sucrose gradient ultracentrifugation to prepare PM microdomains. Twelve fractions were collected from the top of the tube, with fractions 4–6 corresponding to the detergent resistant membrane (DRM) fractions and fractions 10–12 corresponding to high density (HD) fractions. Expression of PKCα, Flotillin (DRM marker) and Calnexin (HD marker) was evaluated by western blot using specific antibodies and revealed by enhanced chemiluminescence. Images are representative of three independent cell culture preparations (n = 3)