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. 2020 Nov 20;10:20251. doi: 10.1038/s41598-020-77190-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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STXM spectra obtained from both all the pixels and specific regions of interest (ROI) within the FIB lamella (see also Fig. S1). Left: Spectra obtained at the C-K edge giving similar results as the EEL spectra, but with a high signal-to-noise ratio and more spectral detail. See text for details of detected bands. Right: N-K edge spectra on these organics showing the two dominant bands due to imine/nitrile C-N bonding environments.