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. 2020 Nov 20;10:20288. doi: 10.1038/s41598-020-77265-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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GD17 uterine CD11b⁺CD45⁺ leukocytes appear to be stress resistant. Flow cytometric analysis of CD11b⁺CD45⁺ cells revealed that stress did not impact uterine (a) polymorphonuclear cell percentages (p = 0.74; neutrophils or PMN-MDSCs), or (b) percent of SSC^low mononuclear cells within the three phenotypes (Ly6C^- alternative M2, p = 0.77; Ly6C^int transitional, p = 0.30; or Ly6C^hi classical M1, p = 0.62). (c) CCR2 MFI within uterine Mo/Mφ/M-MDSCs isolated from restrained dams did not differ from controls (p = 0.13). Data are mean ± SEM, with each dot representing a single dam. NS = non-stressed, n = 6; S = stressed, n = 9; PMN-MDSCs = polymorphonuclear myeloid-derived suppressor cells; Mo/Mφ/M-MDSCs = monocyte/macrophage/monocytic myeloid-derived suppressor cells; MFI = median fluorescence intensity.