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. 2020 Nov 20;11(11):995. doi: 10.1038/s41419-020-03205-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a siRNAs specifically inhibits the expression of ASB16-AS1 in adrenocortical carcinoma cells. 50 nM of ASB16-AS1 siRNA1 (siRNA1) or ASB16-AS1 siRNA2 (siRNA2) were transfected into adrenocortical carcinoma cell SW-13 or H295R, 48 h post transfection the expression of ASB16-AS1 were analyzed by qRT-PCR. *P < 0.05 versus NC group. b Inhibition of ASB16-AS1 expression promoted the proliferation of adrenocortical carcinoma cells analyzed by CCK-8 assay. Adrenocortical carcinoma cells were transfected with ASB16-AS1 siRNA1 (siRNA1) or ASB16-AS1 siRNA2 (siRNA2) at the concentration of 50 nM, cell proliferation was analyzed by CCK-8 assay at the indicated time post-transfection. *P < 0.05 versus NC group. c, d Inhibition of ASB16-AS1 expression promotes the proliferation of adrenocortical carcinoma cells analyzed by EdU incorporation assay. Adrenocortical carcinoma cells were transfected with ASB16-AS1 siRNA1 (siRNA1) or ASB16-AS1 siRNA2 (siRNA2) at the concentration of 50 nM, cell proliferation was analyzed by EdU assay. Scale bar equals 100 μm. *P < 0.05 versus NC group. e, f Inhibition of ASB16-AS1 promotes cell cycle progression in adrenocortical carcinoma cells. Adrenocortical carcinoma cells were transfected with 50 nM of ASB16-AS1 siRNA1 (siRNA1) or ASB16-AS1 siRNA2 (siRNA2), cell cycle was analyzed by FACS assay. *P < 0.05 versus NC group. The experiments were performed in triplicate. The data are represented as mean ± SEM from three independent experiments.