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. 2020 Nov 20;11(11):995. doi: 10.1038/s41419-020-03205-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Heatmap of RNA transcriptome sequencing upon inhibition of ASB16-AS1 expression. SW-13 adrenocortical carcinoma cells were transfected with ASB16-AS1 siRNA2 and RNA sequencing was performed 48 h post-transfection. b Gene ontology (GO) analysis of up-regulated genes when the expression of ASB16-AS1 is inhibited. c, d Inhibition of ASB16-AS1 up-regulates CDK6 and IGF1R mRNAs expression. ASB16-AS1 siRNA1 (siRNA1) or ASB16-AS1 siRNA2 (siRNA2) was transfected into adrenocortical carcinoma cell SW-13 or H295R cells and qRT-PCR was performed to analyze the expression of CDK6 and IGF1R expressions 48 h post transfection. *P < 0.05 versus NC group. e Inhibition of ASB16-AS1 up-regulates the protein levels of CDK6 and IGF1R. ASB16-AS1 siRNA1 (si1) or ASB16-AS1 siRNA2 (si2) was transfected into SW-13 or H295R cells, the protein levels of CDK6 and IGF1R were analyzed by western blot. f Immunohistochemical staining of CDK6 and IGF1R in sections from xenograft tumors stably expressing ASB16-AS1. Scale bar equals 100 μm. The experiments were performed in triplicate. The data are represented as mean ± SEM from three independent experiments.