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. 2020 Nov 20;11:5914. doi: 10.1038/s41467-020-19621-z

Fig. 1. CRWN expression and localization patterns in Arabidopsis thaliana.

Fig. 1

a β-Glucuronidase (GUS) signals in young first leaves (8-day-old plant), adult first leaves (14-day-old plant), and primary roots of pCRWN1::CRWN1-GUS, pCRWN2::CRWN2-GUS, and pCRWN3::CRWN3-GUS. Scale bars = 200 µm (top), 1 mm (middle), and 100 µm (bottom). b Confocal fluorescence images of cotyledons and roots showing pCRWN1::CRWN1-EYFP in crwn1, pCRWN2::CRWN2-EYFP in crwn2, and pCRWN3::CRWN3-sGFP in crwn3. EYFP and sGFP signals are green and propidium iodide signals are magenta. Scale bars = 10 µm (top and middle) and 50 µm (bottom). c Transmission electron micrograph of the nucleus in a root meristematic cell. Lower panel shows a magnified image of the broken square in the upper panel. White arrowheads mark gold particle signals. Dotted and broken lines indicate outer and inner nuclear membranes, respectively. Red, orange, and blue areas indicate the nuclear lamina, internal, and external areas, respectively. Scale bars = 200 nm. d Number of gold particles in each area. Data are mean ± SEM. Significance was determined using unpaired two-sided t-test (n = 5 individual nucleus). Each data point represents a cross mark. e Immunofluorescence images of nuclear surface and nuclear interior. Immunostaining signals are green and DAPI signals are magenta in the merged panel. Scale bar = 5 µm. f Confocal fluorescence images of root epidermal cells in the elongation zone. Scale bar = 5 µm. g STED images of nuclei isolated from 7-day-old seedlings. Lower panels are magnified images of the area indicated by the small square in the upper panels. Scale bars = 5 µm (top) and 1 µm (bottom).