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. 2020 Nov 20;3:699. doi: 10.1038/s42003-020-01432-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Super-resolution imaging of Piwi (green channel) and Dpn (magenta)-positive extensions, which emanate from the cells and end in the brain midline converging to a Piwi-positive cell (indicated with arrow at the middle of the left panel). Also, a smaller Piwi/Dpn+ cell appears to be connected to a larger neighboring cell by the extension (indicated with top arrow). b A super-resolution optical section showing details of an extension’s segment. c A super-resolution optical section of the brain midline showing details of the central segments of the extensions converging in the Piwi-positive cell. Right panels in a–c show the corresponding 3D reconstructions based on super-resolution optical sections (green channel). To visualize extensions, the images were generated with either high laser power during acquisition or post-acquisition by increasing the signal intensity with Imaris software. Scale bars in a, b, and c are 20, 2, and 5 μm respectively.