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. 2020 Nov 20;3:701. doi: 10.1038/s42003-020-01424-z

Fig. 5. Protein kinase activity of GST:PfNek1 fusion proteins.

Fig. 5

a Coomassie gel of wild-type Nek1 incubated with MBP and the indicated inhibitors (PuvB, purvalanol B). b Autoradiogram of the gel in a, showing MBP phosphorylation. c Coomassie gel of wild-type Nek1 (wt), mutant Nek1 (mutations G143D, A246G, M66I and inactive control S200A indicated) and GST-only negative control. d Autoradiogram of the gel in c, showing MBP phosphorylation. Full-length gel and autoradiography for panels c and d are shown in Supplementary Fig. 3.