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. 2020 Nov 20;11(11):997. doi: 10.1038/s41419-020-03213-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a The genomic coordinates of ZNF300 promoter region CpG island and primer locations. The panel plots the GC content as a percentage of the total. Each vertical bar in the bottom panel represents the presence of a CpG dinucleotide. Black horizontal bars indicate regions amplified by targeted bisulfite sequencing primer pairs and RQ-MSP primer pairs. This figure was created using CpGplot (http://emboss.bioinformatics.nl/cgi-bin/emboss/cpgplot) and Methyl Primer Express v1.0 software. TSS: transcription start site; RQ-MSP: real-time quantitative methylation-specific PCR. b The correlation of the candidate gene methylation results between the targeted bisulfite sequencing and RQ-MSP. The correlation was analyzed by Spearman correlation test. c The methylation level of the ZNF300 in larger samples of controls (n = 46), de novo MDS (n = 70) and AML patients (n = 170) analyzed by targeted bisulfite sequencing. P-values were calculated using the Mann–Whitney U-test. d ROC curve analysis using ZNF300 methylation for discriminating AML patients from controls. (e): The impact of ZNF300 methylation on leukemia-free survival and overall survival of MDS and AML patients. Survival was analyzed through Kaplan–Meier analysis using Log-rank test.