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. 2020 Nov 20;11:5919. doi: 10.1038/s41467-020-19670-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Immunofluorescence assays to explore IL-10-induced IL-10RB trafficking to early endosomes. The indicated cells (FL or Nt) were continuously treated with IL-10 (50 ng/ml) for the shown times and fixed for immunofluorescence with anti-Flag (red) and anti-EEA1 (green) antibodies. Shown are representative confocal pictures. Arrows indicate examples of colocalization events. b Quantification of the phenotypes observed in a. The left panel shows the number of Flag-positive vesicles per cell at different times after IL-10 treatment in this experiment, and the right panel represents the percentage of colocalizing red (Flag) and green (EEA1) signals per cell (F: FL; N: Nt). Data are presented as box-plots where the central line represents the median value, the box shows percentiles 25–75 and the whiskers include the most extreme values (n = 27 cells; n.s: P > 0,05; *P < 0.05; **P < 0.01; ****P < 0.0001, two-sided Student’s t-test).