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. 2020 Nov 20;11:5920. doi: 10.1038/s41467-020-19258-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Summary of eRPA test results for detection of RNA from SARS-CoV-2 or from other viruses. Synthetic full genome SARS-CoV-2 RNA was amplified by eRPA using primers targeting the N or S gene and reactions were read out by lateral flow strip. The specificity of eRPA was tested against either in vitro transcribed (IVT) RNA of the related viruses MERS and SARS-CoV, or IVT RNA of the common cold coronaviruses HCoV-HKU1 and HCoV-229E, or viral genomic RNA extracted from 2009 H1N1 Influenza. Data points represent positive (yellow circles) or negative (black squares) eRPA tests for each sample tested and are staggered on both axes for visualization. b Quantification of the synthetic full genome SARS-CoV-2 RNA used as input in the eRPA assay by RT-qPCR. Data are Ct values determined using a one-step commercial RT-qPCR assay using primers targeting either the N or S gene of SARS-CoV-2. Data points at Ct = 40 represent non-specific or no amplification. N gene (orange triangles) and S gene (blue circles) data are offset on the x-axis for visualization purposes. c Lateral flow strip readouts for all N gene data shown in a. Individual strips are labeled with the test call made within 20 min of detection (positive (+) or negative (−)). The positive (Pos.) eRPA control is 1000 copies of synthetic full genome SARS-CoV-2 RNA and the negative (Neg.) eRPA control is a water-only input. Images taken for the purpose of display were allowed to dry which reduced the intensity of some weak bands (labeled with asterisks). Source data are available in the Source Data file.