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. 2020 Nov 20;10:20283. doi: 10.1038/s41598-020-77497-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of DC-SIGN, TLR2 and TLR4 blocking on the expression of maturation markers in ES L1 treated dendritic cells (DCs). Immature DCs were incubated with ES L1 (50 μg/ml) alone, as well as in the presence of specific DC-SIGN blocking antibodies (20 ng/ml) alone or simultaneously with TLR2 and TLR4 blocking antibodies (10 μg/ml). Non-treated cells cultivated in medium were used as a negative control (control) and LPS (200 ng/ml) treated DCs were used as a positive control for complete maturation. After 48 h, the expression of markers (HLA-DR, CD83, CD86, CD40, CCR7 and ILT3) was measured by flow cytometry. (A) Representative analysis of surface markers expression is shown, and (B) the summarized results are shown as mean value of percentage (%) of markers expression ± SD from three different experiments. *p < 0.05, **p < 0.01, ***p < 0.005 as indicated by line (one-way ANOVA with Tukey’s posttest).