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. 2020 Nov 20;10:20283. doi: 10.1038/s41598-020-77497-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of DC-SIGN, TLR2 and TLR4 blocking on the cytokine expression in ES L1-treated dendritic cells (DCs). The analysis of interleukin (IL)-10, IL-12 and transforming growth factor (TGF)-β expression within DCs was performed by intracellular staining, measured by flow cytometry and shown as mean ± SD of the percentage (%) of cytokines expression from three different experiments. *p < 0.05, **p < 0.01, ***p < 0.005 compared to control (non-treated cells cultivated in medium), or as indicated (one-way ANOVA with Tukey’s posttest).