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. 2020 Nov 20;10:20283. doi: 10.1038/s41598-020-77497-x

Figure 4.

Figure 4

Effect of DC-SIGN blocking on the expression of maturation markers in ES L1 treated dendritic cells (DCs). Immature DCs were treated with ES L1 products (50 μg/ml), in the presence or in the absence of specific DC-SIGN blocking antibodies (20 ng/ml), on day 4 of culture for 24 h and then additionally activated or not with LPS for the next 24 h, followed by flow cytometry analysis. (A) Representative analysis of the surface markers expression is shown) (gating strategy is presented in Fig. 2), and (B) the summarized results of percentages of HLA-DR, CD83, CD86, CD40, CCR7 and ILT3 expression by DCs are shown as mean ± SD from three different experiments. *p < 0.05, **p < 0.01, ***p < 0.005 compared as indicated (one-way ANOVA with Tukey’s posttest).