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. 2020 Nov 20;10:20283. doi: 10.1038/s41598-020-77497-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of DC-SIGN on tolerogenic properties and functions of ES L1-treated dendritic cells (DCs) challenged with pro-inflammatory stimulus. DCs treated with ES L1 products (50 μg/ml), in the presence or in the absence of specific DC-SIGN blocking antibodies (20 ng/ml), and then additionally activated or not with LPS, were washed thoroughly and then cocultured with magnetic-activated cell sorting-purified allogenic T cells (Tly) in 1:50 DC:T cell ratio, as described. (A) Representative analysis of CD25+FoxP3+ cells within CD4+ T cells from one experiment is shown, (B) Representative analysis of IL-10 and transforming growth factor (TGF)-β within CD4+CD25+ T cell population is shown, and (C) the summarized results for the expression of CD4+CD25+FoxP3+ T regulatory cells and IL-10 and TGF-β within CD4+CD25+ T cells are shown as the mean% ± SD from three different experiments. *p < 0.05, **p < 0.01, ***p < 0.005 compared as indicated (one-way ANOVA with Tukey’s posttest).