Table 1.
Primers for Construction of KISS1 5′ Deletion
| Name | Sequence | Product (bp) | Accession number |
|---|---|---|---|
| P0(−2261/+ 221) | F: CGCGAGCTCCCTCACTCACCAGCCTGTTTC | 2482 | NM_001134964.1 |
| R: CCCCCGGGGGCCAGCCAGTCTTAGGTTTCCATTA | |||
| P1(−1985/+ 221) | F: CGCGAGCTCGACATCCCTCACTCCCTACTACCC | 2206 | NM_001134964.1 |
| ditto | |||
| P2(−1574/+ 221) | F: CGCGAGCTCTGGGTTTCAGGGTATCACAGAGC | 1795 | NM_001134964.1 |
| ditto | |||
| P3(−1161/+ 221) | F: CGCGAGCTCGGCTCGCCAGTGGTTTATCTTT | 1382 | NM_001134964.1 |
| ditto | |||
| P4(−850/+ 221) | F: CGCGAGCTCTAAGGGTTATGAGAGCAAGCAGGAT | 1071 | NM_001134964.1 |
| ditto | |||
| P5(−289/+ 221) | F: CGCGAGCTCTGCTATTCAGACTCATCCCTCCACT | 510 | NM_001134964.1 |
| ditto |
* The underlined is enzyme-cutting sites