REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-mouse CD16/CD32 Purified	BioXCell	Cat#BE0307; RRID: AB_2736987
anti-mouse CD28 purified	Tonobo	Cat#40-0281-M001; RRID: AB_2621445
anti-mouse Bcl-6 AF 647	BD Biosciences	Cat#561525; RRID: AB_10898007
anti-mouse CCR6 BV785	Biolegend	Cat#129823; RRID: AB_2715923
anti-mouse CD3 AF 594	Biolegend	Cat#100240; RRID: AB_2563427
anti-mouse CD3 APC-Fire750	Biolegend	Cat#100248; RRID: AB_2572118
anti-mouse CD4 PerCP-Cy5.5	Biolegend	Cat#100540; RRID: AB_893326
anti-mouse CD19 BV605	Biolegend	Cat#115540; RRID: AB_2563067
anti-mouse CD21/CD35 (CR2/CR1) BV421	Biolegend	Cat#123422; RRID: AB_2650891
anti-mouse CD38 PE-Cy7	Biolegend	Cat#102718; RRID: AB_2275531
anti-mouse CD44 BV605	Biolegend	Cat#103047; RRID: AB_2562451
anti-mouse CD44 FITC	Biolegend	Cat#103006; RRID: AB_312957
anti-mouse/human CD45R/B220 BV650	Biolegend	Cat#103241; RRID: AB_11204069
anti-mouse/human CD45R/B220 AF 700	eBioScience	Cat#56-0452-82; RRID: AB_891458
anti-mouse CD62L BUV395	BD Biosciences	Cat#740218; RRID: AB_2739966
anti-mouse CD86 BV421	Biolegend	Cat#105032; RRID: AB_2650895
anti-mouse CD138 BV650	BD Biosciences	Cat#564068; RRID: AB_2738574
anti-human/mouse/rat CD278 (ICOS) AF 488	Biolegend	Cat#313514; RRID: AB_2122584
anti-mouse CXCR4 Biotin	eBioScience	Cat#13-9991-82; RRID: AB_10609202
anti-mouse CXCR5 Biotin	eBioScience	Cat#13-7185-82; RRID: AB_2572800
anti-mouse CXCR5 BV421	Biolegend	Cat#145512; RRID: AB_2562128
anti-mouse FAS BV510	BD Biosciences	Cat#563646; RRID: AB_2738345
anti-mouse IgD PE-Cy7	Biolegend	Cat#405720; RRID: AB_2561876
Anti-mouse IgD AF 647	Biolegend	Cat#405708; RRID: AB_893528
anti-mouse IgG1 V450	BD Bioscience	Cat#562107; RRID: AB_10894002
anti-mouse IgG2a/2b BB700	BD Bioscience	Cat#745969; RRID: AB_2743378
anti-mouse IgM FITC	Jackson ImmunoResearch	Cat#115-095-020; RRID: AB_2338592
anti-mouse IFN-g BV650	Biolegend	Cat#505832; RRID: AB_2734492
anti-mouse IL-4 AF 647	Biolegend	Cat#504110; RRID: AB_493322
anti-mouse GL7 PerCP-Cy5.5	Biolegend	Cat#144610; RRID: AB_2562979
anti-mouse PD-1 PE-Cy7	Biolegend	Cat#109110; RRID: AB_572017
anti-mouse Ter119 APC-Fire750	Biolegend	Cat#116250; RRID: AB_2819833
Goat anti-Human IgG Fc Secondary Antibody, PE	eBioScience	Cat#12-4998-82; RRID: AB_465926
goat anti-mouse IgG-HRP	Jackson ImmunoResearch	Cat#115-035-003; RRID: AB_10015289
rat anti-mouse IgG1-HRP	Southern Biotech	Cat#1144-05; RRID: AB_2734757
rat anti-mouse IgG2a-HRP	Southern Biotech	Cat#1155-05; RRID: AB_2794648
rat anti-mouse IgG2b-HRP	Southern Biotech	Cat#1186-05; RRID: AB_2794687
anti-VSV Indiana G	Absolute Antibody	Cat#Ab01401-2.0; RRID: AB_2883992
Chemicals, Peptides, and Recombinant Proteins
ACK Lysing Buffer	Lonza	Cat#10-548E
Cytofix/Cytoperm	BD Biosciences	Cat#554714; RRID: AB_2869008
ELISPOT AEC Substrate Set	BD Biosciences	Cat#551951; RRID: AB_2868954
Expifectamine 293 transfection kit	Gibco	Cat#A14525
Fixable Viability Dye eFluor780	eBioScience	Cat#115-095-020
GolgiPlug	BD Biosciences	Cat#555029; RRID: AB_2869014
Lightning-Link(R) Phycoerythrin	Expedeon	Cat#703-0010
Lightning-Link(R) Alexa Fluor 647	Expedeon	Cat#336-0005
Pierce TMB Substrate Kit	Thermo Scientific	Cat#34021
Recombinant Mouse IL-21R Fc Chimera Protein	R&D Systems	Cat#596-MR-100
Recombinant RBD and and Full Spike	Florian Krammer	N/A
SARS-CoV-2 spike peptide pools	JPT	Cat#PM-WCPV-S
Streptavidin-BV421	Biolegend	Cat#405225
Streptavidin-AF488	Biolegend	Cat#405235
Experimental Models: Cell Lines
HEK293T	ATCC	Cat#CRL11268; RRID: CVCL_1926
FreeStyle 293 (293F) cells	Gibco	Cat#R79007
Experimental Models: Organisms/Strains
Balb/c mouse	The Jackson Laboratory	Stock#000651
C57BL/6J mouse	The Jackson Laboratory	Stock#000664
Balb/c mouse	Charles River Laboratories	N/A
Experimental Models: Bacterial and Virus Strains
SARS-CoV-2 (USA/WA1/2020)	BEI Resources	NR-52281
Oligonucleotides
Actb	Integrated DNA Technologies	Forward 5’- GAGGTATCCTGACCCTGA AGTAReverse 5’- GCTCGAAGTCTAGAG CAACATAG;
Ascl2	Integrated DNA Technologies	Forward 5’-GAGAGCTAAGCCCGATG GAReverse 5’-AGGTCCACCAGGAGT CACC
Software and Algorithms
FlowJo v10	FlowJo LLC	N/A
S6 Fluorospot Analyzer	CTL	N/A
Graphpad Prism v8	Graphpad	N/A
Volocity 6.3	Perkin Elmer	N/A