Table 1. Characterization of microsatellite loci for each Dasypterus taxon examined.

Each locus has the number of individuals (n), allele size range in bp, number of alleles (N_a), allelic richness (A_r), observed heterozygosity (H_O), expected heterozygosity (H_E), and the inbreeding coefficient F_IS. A_r was only calculated using loci shared among all taxa.

Species	Locus	n	Size range (bp)	Na	Ar	HO	HE	FIS
D. ega	Coto_G12	118	211–231	10	–	0.839	0.808	−0.038
	LAS7468	115	311–419	40	4.61	0.974	0.954	−0.021
	LAS8830	117	254–284	13	3.59	0.821	0.822	0.002
	LAS9555	116	441–489	16	–	0.793	0.836	0.051
	Cora_F11	117	146–155	2	–	0.513	0.498	−0.029
	LAS9151	118	262–306	11	2.64	0.551	0.588	0.063
	LAS7831	116	401–423	11	3.76	0.871	0.849	−0.026
	LcM	116	195–241	20	–	0.690	0.712	0.031
	LcU	116	220–253	7	3.14	0.793	0.750	−0.057
D. i. floridanus	Coto_G12	50	211–231	10	–	0.800	0.839	0.047
	LAS7468	49	325–376	20	4.41	0.980	0.927	−0.057
	LAS8830	50	262–276	8	3.19	0.760	0.751	−0.012
	LAS8425	50	166–172	4	–	0.300	0.269	−0.117
	LAS9151	50	272–290	4	2.00	0.520	0.491	−0.059
	LAS7831	50	399–418	10	3.82	0.800	0.854	0.063
	LcM	50	203–245	13	–	0.740	0.803	0.078
	LcU	50	228–253	7	2.99	0.640	0.662	0.033
D. i. intermedius	LAS7468	208	325–384	24	4.37	0.938	0.930	0.932
	LAS8830	211	264–278	8	3.23	0.716	0.764	−0.009
	LAS2547	184	428–461	15	–	0.842	0.827	0.063
	LAS8425	211	166–188	5	–	0.209	0.202	−0.019
	LAS9151	211	264–293	9	2.10	0.507	0.519	−0.032
	LAS7831	205	397–417	11	3.94	0.873	0.876	0.022
	LcU	211	224–228	7	2.99	0.720	0.715	0.004
D. intermedius	LAS7468	257	325–384	37	4.52	0.946	0.946	0.001
	LAS8830	261	262–278	9	3.22	0.724	0.762	0.050
	LAS8425	261	166–188	5	–	0.226	0.215	−0.051
	LAS9151	261	264–293	9	2.08	0.510	0.513	0.008
	LAS7831	255	397–418	12	3.93	0.859	0.876	0.019
	LcU	261	224–253	9	2.96	0.705	0.708	0.004