Table 2. Microsatellite primers and respective species from which they were obtained, used for amplifying sequences of the mothers and the respective offspring for the suspected parthenogenesis cases in Bothrops atrox, B. moojeni, and B. leucurus.

Locus	Species	DNA strand	Primer sequences	Annealing (T °C)	References
Microsatellites
Ac4335	Agkistrodon contortrix	5′	ATC CTT CCC CAA GCC AAG G	62	Castoe et al. (2010)
		3′	GCT GGA GAC TGG AGA AGA GAG C
MR102	Bothrops marmoratus	5′	CTC TTT TGC AGT TAT GGC CC	56	Machado (2015)
		3′	TGG CTT AGG AAG ACA CTG AAA
Bi52.13	B. insularis	5′	TAC TGT ATT GCA CCG GCT AAG G	62; 56a	K Zamudio (pers. comm., 2013)
		3′	AAT CTC CTG TTT TAA TGC TAC TGA A
Bi60.3	B. insularis	5′	CTT TGC CGC CGA TGG TG	60	K Zamudio (pers. comm., 2013)
		3′	GGT TGG GCC TGT GGA CTG TT

Notes.

^aAnnealing temperature varied according to the species: 62 °C for B. moojeni and 56 °C for B. leucurus.