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. 2020 Nov 20;5:21. doi: 10.1038/s41536-020-00101-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Vessels were stained with VEGF (red), and nuclei were stained with DAPI (blue) in the control group, model group, PRP-2.5× group, PRP-4.5× group and PRP-6.5× group. b The statistical analyses of the mean IOD of VEGF on the operated side. c The correlation analysis between AUC of CEUS and the IOD of VEGF. f The correlation analysis between the PI of CEUS and the IOD of VEGF. e CEUS examination of injured nerve on the operated side. The enhanced time was shown in the horizontal axis and the enhanced intensity was shown in the vertical axis. The time-intensity curves (proximal: pink and distal: yellow) of the CEUS obtained for the control group, model group, PRP-2.5× group, PRP-4.5× group and PRP-6.5× group. f Comparisons of the AUC on the CEUS in the operated sciatic nerve among groups. g Comparisons of the PI on the CEUS in the operated sciatic nerve among groups. *P < 0.05, **P < 0.01, and n.s. not significant. Error bars = SD, n = 5. CEUS contrast-enhanced ultrasonography, PRP platelet-rich plasma, IOD integral optical density, AUC area under the curve, PI peak intensity.