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. 2020 Nov 20;11:5921. doi: 10.1038/s41467-020-19097-x

Fig. 3. SARS-CoV-2 detection from unextracted samples using SHINE.

Fig. 3

a Schematic of SHINE, which streamlines SARS-CoV-2 detection by using HUDSON to inactivate samples and single-step SHERLOCK to detect viral RNA with an in-tube fluorescent or colorimetric readout. Times suggested incubation times, C control line, T test line. b Measurement of RNase activity using RNaseAlert after 30 min at room temperature from treated or untreated universal viral transport medium (UTM), saliva, and phosphate-buffered saline (PBS). c SARS-CoV-2 RNA detection in UTM using SHINE with the in-tube fluorescence readout after 1 h. d SARS-CoV-2 RNA detection in saliva using SHINE with the in-tube fluorescence readout after 1 h. e Schematic of the companion smartphone application for quantitatively analyzing in-tube fluorescence and reporting binary outcomes of SARS-CoV-2 detection. f Colorimetric detection of SARS-CoV-2 RNA in unextracted patient NP swabs using SHINE after 1 h. g SARS-CoV-2 detection from 50 unextracted patient samples using SHINE and smartphone application quantification of in-tube fluorescence after 40 min. Threshold line plotted as mean readout value for controls plus 3 standard deviations. h Concordance table between SHINE and RT-qPCR for 50 patient samples. For b, center = mean for 2 technical replicates. For b, g, source data are provided as a Source data file.