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. 2020 Nov 17;13:11807–11818. doi: 10.2147/OTT.S274451

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© 2020 Jin et al.

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Effect of miR-1224-5p on the expression of related proteins of PI3K/AKT/mTOR pathway and EMT. Western blot is applied to detect the expression levels of EMT-related proteins E-cadherin, Vimentin, N-cadherin, ZEB1 (A) and the expression levels of PI3K/AKT/mTOR signal pathway-related proteins p-PI3K, p-AKT, p-mTOR, PI3K, AKT and mTOR (B) in MG-63 cells and U2OS cells after transfection with miR-1224-5p mimic and NC mimic. *P<0.05 vs mi-NC group.