Fig. 1.

pAc/emm55 delays tumor growth in B16 melanoma-bearing mice. B16-bearing mice with palpable tumors (approximately 25 mm2) received three doses of IL injection of pAc/emm55 or pAc/Empty vector on a weekly interval. Invivo-jetPEI transfection reagent (VWR) was mixed with pAc/emm55 or pAc/Empty vector to get homogenous complex for IL injection. Tumor growth was measured and monitored 2–3 times a week. a End point PCR expression on tumor cells transfected with pAc/emm55 in vitro and b qRT-PCR on one representative tumor per group; c tumor weight *p < 0.05 Student’s t test. d Tumor growth curves, CGGC permutation test. e Survival curves, log-rank test. Data represent mean + SEM, N = 12 per group