Figure 6.

TBK1 controls the expression of Mt1 in IECs. (A) A Heat map of highly variable genes detected in RNA sequencing analysis using primary IECs from Tbk1^WT and Tbk1^IEC-KO mice (n = 3 each). (B) qRT-PCR analysis of the indicated mRNAs in freshly isolated IECs from Tbk1^WT and Tbk1^IEC-KO mice (each circle represents a mouse). Data are presented as values relative to Actb. (C) Immunoblot analysis of the indicated proteins in small intestinal IECs of Tbk1^WT and Tbk1^IEC-KO mice. (D and E) qRT-PCR analysis of Mt1 mRNA expression relative to Actb (D) and immunoblot analysis of MT1 protein expression (E) in freshly isolated IECs derived from the large intestines of Tbk1^WT and Tbk1^IEC-KO mice. (F) Immunoblot analysis of the indicated proteins in the nuclear or cytoplasmic extracts of freshly isolated small intestine IECs of Tbk1^WT and Tbk1^IEC-KO mice. Data are representative of 1 (A), or 2 (D-F), or 3 (B and C) independent experiments. Summary data are mean ± s.e.m. with P values being determined by unpaired two-tailed Student’s t-test. **P < 0.01, ***P < 0.001; ns, not significant.