KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-ADRM1 (hRpn13) | Novus Biologics | Cat#NBP1–30447; RRID: AB_2225663 |
| Rabbit monoclonal anti-p21 waf1/cip1 (12D1) | Cell Signaling Technology | Cat#CST #2947; RRID: AB_823586 |
| Rabbit polyclonal anti-p27/kip1 | Novus Biologics | Cat#NBP1–32213; RRID: AB_10003918 |
| Mouse monoclonal anti-GAPDH 6C5 | Santa Cruz Biotechnology | Cat#Sc-32233; RRID: AB_627679 |
| Rabbit polyclonal anti-20S proteasome beta 5 subunit | Enzo Life Sciences | Cat#BML-PW8895–0025; RRID: AB_2052392 |
| Rabbit polyclonal anti-USP14 | Bethyl Laboratories | Cat#A300–920A; RRID: AB_2304343 |
| Rabbit monoclonal anti-UCH37 [EP4897] | Abcam | Cat#Ab133508 ; RRID: AB_2814821 |
| Monoclonal anti-FLAG M2 | Sigma-Aldrich | Cat#F1804; RRID: AB_262044 |
| Goat anti-rabbit 800CW | Licor | Cat#925–32211; RRID: AB_2651127 |
| Donkey anti-mouse 680RD | Licor | Cat#925–32212; RRID: AB_27116622 |
| Mouse monoclonal Anti-β-tubulin | Santa Cruz Biotechnology | Cat#SC-5274; RRID: AB_2288090 |
| Rabbit polyclonal anti-Rpn2 | Novus Biologicals | Cat#NBP1–58221; RRID: AB_11041020 |
| Bacterial and Virus Strains | ||
| BL21(DE3)pLysS competent cells | Promega | Cat#L1195 |
| XL-10 Gold Ultracompetent cells | Agilent | Cat#200314 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| KDT-11 | Synthesized as reported | Trader, et al. 2015 |
| KPD-11 | This paper | N/A |
| KPD-12 | This paper | N/A |
| Ubiquitin-AMC | Boston Biochem | Cat#U-550 |
| Ubiquitin-fluorescein | Prepared as reported | Du, et al. 2017 |
| Omnifect transfection reagent | Transomic | Cat#OTR1003 |
| Ubiquitin (from bovine erythrocytes) | Sigma | CAS: 79586–22-4 Cat#: U6253 |
| bAP-15 | Cayman Chemical | Cat#: 11324 |
| Propidium iodide | Sigma Aldrich | Cat#P4864 |
| Critical Commercial Assays | ||
| CellTiter-Glo 2.0 Cell Viability Assay | Promega | Cat#G9241 |
| Caspase-Glo 3/7 Assay | Promega | Cat#G8090 |
| CellTox Green Cytotoxicity Assay | Promega | Cat#G8741 |
| Click-iT EdU Alexa Fluor 647 Flow cytometry assay | Life Technologies | Cat#C10424 |
| Proteasome purification kit | Enzo Life Sciences | Cat#BML-PW1075A-0001 |
| Experimental Models: Cell Lines | ||
| MM1.R myeloma cell line, Homo sapiens | ATCC | Cat#CRL-2975; RRID: CVCL_8794 |
| SK-MEL-5 melanoma cell line, Homo sapiens | ATCC | Cat#HTB-70; RRID: CVCL_0527 |
| HEK293T kidney cell line, Homo sapiens | ATCC | Cat#CRL-3216; RRID: CBCL_0063 |
| MM1.S myeloma cell line, Homo sapiens | ATCC | Cat#CRL-2974; RRID: CVCL_8792 |
| Oligonucleotides | ||
| ON-TARGETplus Rpn13 (11047) siRNA SMARTpool | Dharmacon | Cat#L-012340–01 |
| ON-TARGETplus GAPDH siRNA SMARTpool | Dharmacon | Cat#D-001830–10 |
| ON-TARGETplus nontargeting siRNA pool | Dharmacon | Cat#D-001810–10 |
| ON-TARGETplus UCHL5/Uch37 (51377) siRNA pool | Dharmacon | Cat#L-006060–00-0020 |
| ON-TARGETplus Usp14 (9097) siRNA pool | Dharmacon | Cat#L-006065–00-0005 |
| Custom ADRM1 (Rpn13) duplex ON-TARGET siRNA - UTR1 Rpn13 Sense:5’AGGAAGAGCGAGCCCGGACUU3’ Antisense:5’GUCCGGGCUCGCUCUUCCUUU3’ |
Dharmacon | Cat#CTM-485557 |
| CustomADRM1(Rpn13)duplexON-TARGETsiRNA- UTR2 Rpn13 Sense: 5’ UCCGAGGAACUGGGCGCUUUU 3’ Antisense: 5’ AAGCGCCCAGUUCCUCGGAUU 3’ |
Dharmacon | Cat#CTM-485558 |
| Site directed mutagenesis primers: For: 5’ CCTCGCCCCAGTTCGCGGCGGCCCTGGGCATGTTCAGCGCAG 3’ | IDT | N/A |
| Site directed mutagenesis primers: Rev: 5’ CATGCCCAGGGCCGCCGCGAACTGGGGCGAGGTCAGGGTATTC 3’ | IDT | N/A |
| Recombinant DNA | ||
| pcDNA5 FLAG-ADRM1 | Yao, et al 2006 | Addgene#19417; RRID: Addgene_19417 |
| Pet19b His6 ADRM1 | Yao, et al 2006 | Addgene #19423; RRID: Addgene_19423 |
| Mammalian expression FLAG-Rpn2(938–952) | Kylie Walters lab National Cancer Institute |
Lu, et al. 2015 |
| Bacterial expression GST-Rpn2(938–952) | Kylie Walters lab National Cancer Institute |
Lu, et al. 2015 |
| pVP16 MBP-Uch37 | DNASU | ID#84019 |
| Software and Algorithms | ||
| ImageJ | https://imagej.nih.gov/ij/ | RRID: SCR_003070 |
| Licor Image Studio Software | LI-COR Image Studio Software | RRID: SCR_015795 |
| Prism 8 | Graphpad | RRID:SCR_000306 |
| Geneious | Geneious | RRID: SCR_010519 |
| ChemDraw | PerkinElmer | RRID: SCR_016768 |
| FlowJo | FlowJo | RRID: SCR_008520 |
| Other | ||
| Amylose resin | NEB | Cat#E8021S |
| Ni-NTA resin | Qiagen | Cat#30210 |
| Pierce Glutathione agarose | Thermo Fisher | Cat#16100 |
| 4–20% Mini-PROTEAN Gels | Biorad | Cat#4561096 |
| Fetal Bovine Serum, Heat inactivated | Life technologies | Cat#10082147 |
| KOD HotStart PCR Master Mix | EMD Millipore | Cat#71842 |
| Mycoplasma PCR Test Kit | ABM Good | Cat#G238 |
| Annexin V BV510 Reagent | BioLegend | Cat#640937 |