qPCR analysis of MR1 gene expression in A. 8D1A and BV2 cell lines, primary purified astrocyte and microglia cultures, and B. brain tissue. The housekeeping gene, GAPDH, was used as an internal control. RNA isolated from spleen-derived B cells and intact spleen tissue was used as a calibrator to determine the level of MR1 gene expression in A and B, respectively. The data are presented as the mean ± SD from two independent cDNA samples with three replicates from each sample.