Fig. 6.

Activation of MAIT cells by E. coli-stimulated astrocytes and microglia. A. 8D1A and BV2 cell lines, B. Primary cultures of astrocytes and microglia, and C. Single cell suspensions from the brains of wild type (WT) and MR1 KO mice, were stimulated for 6 h with PBS or PFA-fixed E. coli at an MOI of 300. The cells were washed three times with PBS and incubated with either an MR1 blocking antibody or isotype control for 1 h, followed by the addition of the murine MAIT cell hybridoma, 8D12. As a control, the E. coli-stimulated target cells and MAIT cells were cultured alone. Following 48 h of culture, the supernatants were harvested and IL-2 production was measured by ELISA. The IL-2 level is expressed in ng/ml. A. & B. Each data point represents the mean of triplicate wells from one of three independent experiments. The data are inclusive of all three independent experiments and the error bars represent the mean ± SD. C. The data shown are from two combined, independent experiments with an N=6 for WT and N=4 for MR1KO mice. n.s, not significant; *, p< 0.05, **, p< 0.005, ***, p<0.0005; ****, p<0.0001; unpaired Student’s t-test.