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. Author manuscript; available in PMC: 2021 Nov 19.
Published in final edited form as: Cell Chem Biol. 2020 Aug 27;27(11):1371–1382.e6. doi: 10.1016/j.chembiol.2020.08.007

Figure 5. MS-Based Analysis of RA190-Protein Interactions in MM1.R Cells.

Figure 5.

(A) Schematic of the label-free quantitative proteomic workflow. See text for details.

(B) Label-free quantitation (LFQ) ratios of proteins from cells treated with 5 μM RA190 or DMSO.

(C) Table of proteins found to be most highly engaged by RA190 and the LFQ ratios of the signals in the RA190- and DMSO-treated samples (a low ratio represents a high level of interaction with RA190 in cellulo). Biological triplicates were analyzed in experimental duplicate, as indicated by standard deviations (represented as ±). Stringency filters were applied to remove potential false positive results. Proteins in green indicate the targets are within the 0.5 cutoff for positive hit identification.