| Biological Samples |
|
|
| Human plasma, single donor |
Innovative Research |
Cat#IPLASNAH2ML |
| Human buffy coat |
Stanford Blood Center |
N/A |
| UltraPool human liver microsomes |
BD |
Cat#452117 |
| Male mouse microsomes (CD-1) |
BD |
Cat#452701 |
| Chemicals, Peptides, and Recombinant Proteins |
| FreeStyle 293 Expression Medium |
ThermoFisher |
Cat#12338018 |
| TARGET peptide (YPGQx5 + V) |
custom synthesis by Stanford PAN facility |
N/A |
| DMEM |
Corning |
Cat#MT10013CV |
| RPMI 1640 |
Corning |
Cat#10-040-CV |
| FBS |
Atlanta Biologics |
Cat#S11150
|
| Penicillin/streptomycin |
ThermoFisher |
Cat#15140163 |
| HEPES (1 M) |
Gibco |
Cat#15-630-080 |
| 2’3’-cGAMP |
This paper |
N/A |
| Recombinant sscGAS |
(Ritchie et al., 2019) |
N/A |
| [α-32P]ATP, 250 μCi |
PerkinElmer |
Cat#BLU003H250UC |
| GTP, disodium salt hydrate |
Sigma Millipore |
Cat#G8877-1G |
| Herring testes DNA |
Sigma Millipore |
Cat#D6898-1G |
| HP-TLC silica gel aluminum |
EMD Millipore |
Cat#1.05548.0001 |
| Human serum albumin |
Sigma Millipore |
Cat#A3782-500MG |
| polyphosphate:AMP phosphotransferase (PAP) |
(Mardjuki et al., 2020) |
N/A |
| Myokinase |
Sigma Millipore |
Cat#M3003-2.5KU |
| Mouse ENPP1 |
This paper |
N/A |
| 2-mercaptoethanol |
Fisher Scientific |
Cat#AC125472500 |
| Human GM-CSF |
PeproTech |
Cat#300-03-5ug |
| Compounds 1–62, detailed synthesis described in Supplemental Information, Data S1 |
This paper |
N/A |
| Critical Commercial Assays |
| CellTiterGlo |
Promega |
Cat#G8461 |
| Deposited Data |
| ENPP1-compound 15 structure |
This paper |
PDB: 6XKD |
| ENPP1-AMP structure |
(Kato et al., 2012) |
PDB: 4GTW |
| Experimental Models: Cell Lines |
| HEK293S GnT1− stably expressing mouse ENPP1 |
(Kato et al., 2012) |
N/A |
| E0771 |
CH3 BioSystems |
Cat#940001 |
| Caco-2 |
ATCC |
Cat#HTB-37 |
| Software and Algorithms |
| Prism 7.03 |
Graphpad |
https://www.graphpad.com/scientific-software/prism/ |
| Mnova 14.1 |
Mestrelab |
https://mestrelab.com/software/mnova/ |
| ChemDraw Professional 18.0 |
PerkinElmer |
https://www.perkinelmer.com/category/chemdraw |
| ImageJ |
(Schneider et al., 2012) |
https://imagej.nih.gov/ij/ |
| Mosflm |
(Leslie and Powell, 2007) |
https://www.mrc-lmb.cam.ac.uk/harry/imosflm/ver721/imosflm_link/ver730/introduction.html |
| CCP4 |
(Winn et al., 2011) |
http://www.ccp4.ac.uk/ |
| SCALA |
(Evans, 2011) |
http://www.ccp4.ac.uk/html/scala.html |
| REFMAC |
(Murshudov et al., 1997) |
http://www.ccp4.ac.uk/html/refmac5.html |
| COOT |
(Emsley et al., 2010) |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Procheck |
(Vaguine et al., 1999) |
https://www.ebi.ac.uk/thornton-srv/software/PROCHECK/ |
| Pymol |
(DeLano, 2002) |
https://www.pymol.org/2/ |
| Other |
| Superose 12 10/300 GL |
GE Healthcare |
GE17-5173-01 |
| Rapid Equilibrium Device |
Thermo Fisher |
Cat#99006 |
