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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biochim Biophys Acta Mol Cell Res. 2020 Oct 2;1868(1):118867. doi: 10.1016/j.bbamcr.2020.118867

Figure 7: Phenotypic analysis of mitochondrial Cu transporters.

Figure 7:

Cu-transporting MCF proteins were identified using genetic screens based on limiting Cu availability with the addition of Ag as a competitor. At concentrations of 150 μM, Ag inhibits Cu uptake and results in decreased COX activity. To elicit the same defect in cells lacking PIC2 only 75–100 μM Ag is required, suggesting a loss of high-affinity transport. Mrs3 was identified as a lower affinity Cu transporter based on additive phenotypes. Yeast cells lacking PIC2 and MRS3 grown under mild Cu chelation without the addition of Ag had decreased COX, decreased IM-SOD1 activity and lower mitochondrial Cu uptake and accumulation.