Figure 1.

Analysis of differentiation indices in myoblasts from male (n=5; black) and female (n=5; grey) rhesus macaques after 5 days of differentiation with or without 50mM ethanol. Myoblast fusion index (A), myotubes per field (B), and total nuclei (C) for ethanol-treated myoblasts expressed as percent of control (0 mM ethanol; dashed line); representative images (20x) of HEMA 3-stained myoblasts (D-G). Values calculated from the average of 10–15 images. *p<0.05, ***p<0.001 analyzed by two-way (sex × condition) ANOVA with repeated measures on condition; mean ± SE.