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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Alcohol Clin Exp Res. 2020 Oct 3;44(11):2166–2176. doi: 10.1111/acer.14453

Figure 3.

Figure 3.

Extracellular acidification rate (ECAR) and ECAR/oxygen consumption rate (OCR) ratio results from Mitochondrial Stress Test using Seahorse XFe24 technology. Baseline ECAR (A) and OCR/ECAR (B); post-oligomycin ECAR (C) and OCR/ECAR (D) in myoblasts from male (n=5; black) and female (n=5; grey) rhesus macaques after 3 days of proliferation with (open circles) or without (closed circles) 50mM ethanol. Cells were seeded at 75,000 cells/well and counted after the assay using a Cytation 5 after staining nuclei using Hoescht dye. OCR and ECAR values are adjusted for cell count. **p<0.01 analyzed by two-way (sex × condition) ANOVA with repeated measures on condition; expressed as individual changes.

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