Figure 5.

DNA sequence and gene expression variation of BnaFLC.A02 in Cabriolet and Darmor. (a) The polymorphisms identified at BnaFLC.A02 in Cabriolet compared with Darmor are highlighted with black arrows, grey boxes represent exons, black dashed lines represent introns, and the black horizontal arrow represents the direction of transcription. (b) Normalized expression of BnaFLC.A02 in Cabriolet, Darmor and genotyped F₂ lines for BnaFLC.A02 and BnaFT.A02, as measured by quantitative RT‐PCR before (NV) and after a six‐week vernalization treatment (6WT0, 6WT16, 6WT32), error bars denote one standard error around the mean calculated from at least three biological replicates. (c) Normalized expression of BnaFLC.A02 in Cabriolet, Darmor and genotyped F₂ lines for BnaFLC.A02 and BnaFT.A02, as measured by quantitative RT‐PCR before (NV) and after a six‐week vernalization treatment (6WT0, 6WT16, 6WT32), error bars denote one standard error around the mean calculated from at least three biological replicates.