Figure 1. Skeletal-Muscle-Specific CPT2-Deficient Mice Accumulate LCACs.

(A) mRNA and protein expression of Cpt2 in skeletal muscle of Cpt2^Sk+/+ and Cpt2^Sk−/− mice (n = 6).

(B–E) Complete and incomplete oxidation of ¹⁴C-palmitate, ¹⁴C-octanoate, and ¹⁴C-pyruvate in skeletal muscle homogenates of Cpt2^Sk+/+ and Cpt2^Sk−/− mice (n = 3).

(F) Acylcarnitine quantification in TA muscle and plasma of Cpt2^Sk+/+ and Cpt2^Sk−/− female mice (n = 6).

(G and H) Ion intensity per microgram of protein of LCACs and of medium- and short-chain ACs in skeletal muscle of Cpt2^Sk+/+ and Cpt2^Sk−/− female mice, 24 weeks of age (n = 6).

(I and J) Ion intensity per microliter of plasma of LCACs and of medium- and short-chain ACs in plasma of Cpt2^Sk+/+ and Cpt2^Sk−/− female mice, 24 weeks of age (n = 6).

(K) Body weight and length of Cpt2^Sk+/+ and Cpt2^Sk−/− male mice, 12 weeks of age (n = 7–12).

(L) Spontaneous, home-cage locomotor activity during light and dark cycles of male mice, 12 weeks of age (average of 2 recording days, n = 8).

(M) Voluntary wheel activity during light and dark cycles of male mice, 12 weeks of age (average of 2 recording days, n = 8).

Data are presented as mean ± SEM. *p < 0.05 determined by Student’s t test. See also Figure S1.