Figure 2. Cpt2^Sk−/− Protection from Obesity Induced by a High-Fat Diet.

(A) Body weight of Cpt2^Sk+/+ and Cpt2^Sk−/− female mice fed a low-fat (LF) or a high-fat (HF) diet for 16 weeks (n = 7–12).

(B) Whole-body fat mass after 2, 5, 10, and 14 weeks of HF diet fed to female mice (n = 7–12).

(C) Wet tissue mass of BAT, iWAT, and gWAT individual depots after 16 weeks of HF diet fed to female mice (n = 7–12).

(D) Representative histological image of gWAT after 8 weeks of HF diet, H&E stain. Scale bar, 100 μm.

(E) Whole-body lean mass after 2, 5, 10, and 14 weeks of HF diet fed to female mice (n = 7–12).

(F) Wet tissue mass of liver, heart, GA, TA, and SOL muscle after 16 weeks of HF diet fed to female mice (n = 7–12).

(G) Food and water intake at 2 and 14 weeks of HF diet fed to female mice (n = 7–12).

(H) Fecal TAGs of male mice after 8 weeks of LF- and HF-diet feeding (n = 5).

(I) Fecal LCACs of male mice after 8 weeks of LF- or HF-diet feeding (n = 5).

(J) Urinary levels of LCACs before and after a 10-day-long HF-diet feeding of male mice (n = 6).

(K) Distribution of the most abundant hydroxylated and dicarboxylated short- and medium-chain species in male mice before and after a 10-day-long HF-diet feeding (n = 6).

(L) Liver, muscle, and BAT mRNA abundance of genes involved in regulation of systemic metabolism of female mice fed a LF or HF diet for 16 weeks (n = 6).

(M) Plasmatic GDF15 of male mice fed a LF or HF diet for 8 weeks (n = 6).

(N and O) Energy expenditure (EE) at 14 weeks of LF- or HF-diet feeding presented as both non-normalized and normalized to body lean mass of female mice (n = 3–5).

(P) Regression plot of EE versus body weight (BW) at 14 weeks of HF-diet feeding. Each regression line represents the ANCOVA predicted mean EE per value of BW (n = 8).

(Q) Resting energy requirements (RERs) at several time points during LF- or HF-diet feeding of female mice (n = 5–10).

Data are presented as mean ± SEM. *p < 0.05 determined by Student’s t test for Cpt2^Sk+/+ HF diet versus Cpt2^Sk−/− HF diet; ^$p < 0.05 for Cpt2^Sk+/+ LF diet versus Cpt2^Sk+/+ HF diet. Statistical analysis by 2-way ANOVA for 4 groups and by ANCOVA for EE. Means depicting a different letter indicate significant differences between groups (p < 0.05) (K–M). See also Figures S1 and S2.