Figure 7. Carnitine Supplementation Improves Systemic Metabolism and Insulin Sensitivity in Cpt2^Sk−/− Mice.

(A) Plasma glucose of Cpt2^Sk+/+ and Cpt2^Sk−/− female mice fed a LF or HF diet supplemented with L-carnitine for 16 weeks (n = 12).

(B) Plasma triacylglycerol of female mice fed a LF or HF diet with L-carnitine (n = 12).

(C) Plasma free fatty acids of female mice fed a LF or HF diet with L-carnitine (n = 12).

(D) Plasma β-OH of female mice fed a LF or HF diet with L-carnitine (n = 12).

(E) Liver mRNA relative abundance of female mice fed a LF or HF diet with L-carnitine. Data are normalized to the −Carn respective group (n = 3–4).

(F) Glucose tolerance test of female mice fed a LF or HF diet with and without L-carnitine (n = 6 for −Carn and n = 3–4 for +Carn).

(G) Insulin tolerance test of female mice fed a LF or HF diet with and without L-carnitine (n = 6 for −Carn and n = 3–4 for +Carn).

(H) Phosphorylation status of Akt protein in TA muscle of female mice fed a LF or HF diet with and without L-carnitine (n = 6 for −Carn and n = 3–4 for +Carn).

Data are presented as mean ± SEM. ^#p < 0.05 between the −Ins versus the +Ins group for each genotype and diet determined by Student’s t test; *p < 0.05 between +Carn versus −Carn for each genotype and diet determined by Student’s t test. Statistical analysis by 2-way ANOVA. Means depicting a different letter indicate significant differences between groups (p < 0.05).