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. 2020 Nov 17;13:11795–11806. doi: 10.2147/OTT.S274492

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© 2020 Yang et al.

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ZFAS1 interacts with miR-647 in CC. (A) Anti-AGO2 RIP was carried out to detect the ZFAS1 associated with AGO2. (B) miR-647 and its putative binding sequence in ZFAS1. The mutant miR-647 binding site in ZFAS1 was also generated (ZFAS1-mut). (C) miR-647 significantly suppressed the luciferase activity that carried wild-type ZFAS1 but not ZFAS1-mut in Caski cells. (D) The MS2-RIP was performed to detect the miR-647 associated with ZFAS1. (E) The biotin-labeled miRNA pull-down assay was performed to detect the ZFAS1 associated with miR-647. (F) The miR-647 expression in control and ZFAS1 knockdown Caski cells. (G) The miR-647 expression in control and ZFAS1 overexpressing Hela cells. (H) The ZFAS1 expression in Caski and Hela cells transfected with miR-NC or miR-647 mimics. *p<0.05, **p<0.01, ***p<0.001.