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. 2020 Nov 18;14:5077–5085. doi: 10.2147/DDDT.S275148

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© 2020 Wang et al.

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Acacetin inhibits cell proliferation in OS cells. (A) Acacetin inhibits human osteosarcoma cell proliferation. 143B, MG63, SJSA and HOS cells were treated with acacetin (0, 15, 30, 45, and 60 μM) for the indicated times (24, 48 and 72 h), followed by assessment of the inhibition rate by the CCK-8 assay (n=3). (B) After the SJSA and HOS cells were treated with 40mL of acacetin for the indicated times (24, 48 and 72 h), the cell viability was measured with CCK-8 assay and compared with the doxorubicin. (C) SJSA, HOS and osteoblast cells were treated with 40 μM acacetin for the indicated times (24, 48 and 72 h) or treated with increased concentrations of acacetin for 48 h and the cell viability was measured with CCK-8 assay. (D) Colony-formation assay of SJSA and HOS cells treated with the DMSO or acacetin. The histogram shows the ratio of clone formation. Control group was normalized to 100%. *P<0.05, significantly different compared with control.