Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 18;14:5077–5085. doi: 10.2147/DDDT.S275148

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Wang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Acacetin induces apoptosis of OS cells. (A) Acacetin induced apoptosis in human osteosarcoma cells. Apoptotic nuclear morphological changes were evaluated by Hoechst 33258 staining and observed under a fluorescence microscope. (B) Acacetin induces apoptosis in HOS and SJSA cells. HOS and SJSA cells were treated with acacetin (0, 40 and 60 μM) for 24 h, and apoptotic cells were analyzed by flow cytometry. The histograms indicate that the percentage of total apoptosis. Results are shown as mean±S.D. from three independent experiments. (C) The mitochondrial membrane potential after acacetin treatment was measured using JC-1 staining by flow cytometry. The histograms indicate the ratio of green and red fluorescence. The results are shown as the mean±S.D. from three independent experiments. (D) Cells were treated with various concentrations of acacetin for 24 h. The apoptosis-related proteins cleaved PARP, caspase-3, −8, and −9, Bax and Bcl-2 were analyzed by Western blotting. *P < 0.05, significantly different compared with the untreated control group.