Table 3.
Patient tumor and matched normal tissue, paired with tissue from different, sequential passages of the corresponding PDX models was sequenced using the TruSeq Amplicon – Cancer Panel. 212 amplicons in 48 oncogenes were targeted. The results obtained for the main oncogenes are summarized in the table (AA = amino acid, n.a. = not analyzed).
Mutation detected, (AA mutation) |
|||||||
---|---|---|---|---|---|---|---|
Patient or PDX, passage | APC | BRAF | EGFR | KRAS | MET | PIK3CA | TP53 |
Co9587, normal tissue | - | - | - | - | - | - | - |
Co9587, patient tumor | n.a. | n.a. | n.a. | G12D | n.a. | n.a. | n.a. |
Co9587, P0 | ins1554;R876 | - | - | G12D | - | del104 | - |
Co9587, P1 | ins1554;R876X | - | - | G12D | - | del104 | - |
Co9587, P2 | ins1554;R876X | - | - | G12D | - | del104 | - |
Co9587, P3 | ins1554;R876X | - | - | G12D | - | del104 | - |
Co9587, P4 | ins1554;R876X | - | - | G12D | - | del104 | - |
Co9775 normal tissue | - | - | - | - | - | - | - |
Co9775, patient tumor | - | - | - | G12D | - | - | G245S |
Co9775, P0 | - | - | - | G12D | - | - | G245S |
Co9775, P1 | - | - | - | G12D | - | - | G245S |
Co9775, P3 | - | - | - | G12D | - | - | G245S |
Co9775, P4 | - | - | - | G12D | - | - | G245S |
Co10925 normal tissue | - | - | - | - | - | - | - |
Co10925,patient tumor | E1379X | - | - | - | - | - | - |
Co10925, P1 | E1379X | - | - | - | - | - | - |
Co10925, P2 | E1379X | - | - | - | - | - | - |
Co10925, P4 | E1379X | - | - | - | - | - | - |