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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: J Invest Dermatol. 2020 May 23;141(1):142–151.e6. doi: 10.1016/j.jid.2020.05.080

Figure 2.

Figure 2.

Regression of α3β1-deficient tumors is accompanied by reduced cell proliferation and increased apoptosis. K14CreERT:α3flx/flx mice were subjected to two-step tumorigenesis, then treated with 4OHT or vehicle, as in Figure 1e. (a) Paraffin-sectioned tumors stained with anti-Ki67 and hematoxylin counterstain. (b) Graph shows % Ki67-positive area relative to total papilloma area. (c) Tumor cryosections stained with anti-K14 (red) and TUNEL (green); indicated regions enlarged 2.3X below each panel. (d,e) Graphs, % TUNEL+ area relative to (d) epithelial area or (e) stromal area. (a,c) t, tumor cells; s, stroma; k, keratinized region; scale bars, 100 μm. (b,d,e) 4OHT-treated tumor data are normalized to vehicle-treated papillomas (dotted line); n≥10 tumors per treatment group, per timepoint. Data are mean +/− s.e.m.; two-tailed t-tests; *P<0.05.