Table 1.
rRNA gene quantification and 16S/ITS rRNA gene region sequencing of fecal samples.
| 10 Days | 3 Months | 1 Year | 2 Years | Total | |
|---|---|---|---|---|---|
| All fecal samples (count) | 274 | 246 | 247 | 248 | 1,015 |
| Detected bacterial DNA (16S rRNA gene region) | 266 (97%) | 243 (99%) | 247 (100%) | 243 (98%) | 999 (98%) |
| Sequenced 16S rRNA V3–V4 gene region amplicons (after rarefaction)a | 178 (65%) | 193 (78%) | 216 (87%) | 170 (69%) | 757 (75%) |
| Detected fungal DNA (ITS rRNA gene region) | 153 (54%) | 148 (60%) | 163 (66%) | 189 (76%) | 653 (64%) |
| Sequenced ITS gene region amplicons (after rarefaction)a | 15 (6%) | 4 (2%) | 7 (3%) | 11 (4%) | 37 (4%) |
a
Samples were sequenced if the qPCR cycle threshold was <35 cycles to provide trustworthy results in the sequencing procedure. Few samples were excluded due to rarefaction.